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reporter vector pcpgfree promoter lucia  (InvivoGen)


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    Structured Review

    InvivoGen reporter vector pcpgfree promoter lucia
    Reporter Vector Pcpgfree Promoter Lucia, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcpgfree+promoter+lucia+vector/pCpGfree-promoter-Lucia/pmc12969294-108-6-10
    Average 94 stars, based on 42 article reviews
    reporter vector pcpgfree promoter lucia - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The anxiety and ethanol intake controlling GAL5.1 enhancer is epigenetically modulated by, and controls preference for, high-fat diet
    Article Snippet: .. The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs) as described in manufacturer’s instructions. .. Methylated and unmethylated plasmids were transfected into SHSY-5Y cells vector using Jetprime transfection reagent (Polyplus-transfection) and incubated for 24 h. Cells were lysed using passive lysis buffer and the activity of the enhancer assessed using a Lucia QUANTI-Luc Gold Assay #3 luciferase kit (InvivoGen).

    Article Title: The anxiety and ethanol intake controlling GAL5.1 enhancer is epigenetically modulated by, and controls preference for, high fat diet
    Article Snippet: .. The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs) as described in manufacturer’s instructions. .. Methylated and unmethylated plasmids were transfected into SHSY-5Y cells vector using Jetprime transfection reagent (Polyplus-transfection) and incubated for 24 hours.

    Article Title: EGR1 recruits TET1 to shape the brain methylome during development and upon neuronal activity
    Article Snippet: .. After enzymatic digestion for at least 4 h, PCR-amplified products were cloned into pCpGfree-promoter-Lucia Vector (Invivogen). ..

    Plasmid Preparation:

    Article Title: The anxiety and ethanol intake controlling GAL5.1 enhancer is epigenetically modulated by, and controls preference for, high-fat diet
    Article Snippet: .. The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs) as described in manufacturer’s instructions. .. Methylated and unmethylated plasmids were transfected into SHSY-5Y cells vector using Jetprime transfection reagent (Polyplus-transfection) and incubated for 24 h. Cells were lysed using passive lysis buffer and the activity of the enhancer assessed using a Lucia QUANTI-Luc Gold Assay #3 luciferase kit (InvivoGen).

    Article Title: The anxiety and ethanol intake controlling GAL5.1 enhancer is epigenetically modulated by, and controls preference for, high fat diet
    Article Snippet: .. The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs) as described in manufacturer’s instructions. .. Methylated and unmethylated plasmids were transfected into SHSY-5Y cells vector using Jetprime transfection reagent (Polyplus-transfection) and incubated for 24 hours.

    Article Title: Sulforaphane epigenetically demethylates the CpG sites of the miR-9-3 promoter and reactivates miR-9-3 expression in human lung cancer A549 cells
    Article Snippet: .. The PCR products were digested with Nsi I and Spe I and then inserted into the pCpGfree-promoter-Lucia vector (InvivoGen, San Diego, CA, USA). .. After sequencing confirmation, the recombinant plasmid was treated with methyltransferase M.Sss I at 37°C for 2 h (New England Biolabs, Beverly, MA, USA).

    Article Title: Epigenetic modifications but not genetic polymorphisms regulate KEAP1 expression in colorectal cancer.
    Article Snippet: People's Republic of China Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, People’s Republic of China Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, New Jersey Department of Laboratory Medicine, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, People’s Republic of China

    Article Title: YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesis.
    Article Snippet: 1Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA.. 2Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.. ✉e-mail: duojia.pan@utsouthwestern.edu The preexisting epigenetic landscape represents a barrier to oncogene-induced cellular transformation and malignancy.

    Article Title: KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2α.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    CpG Methylation Assay:

    Article Title: The anxiety and ethanol intake controlling GAL5.1 enhancer is epigenetically modulated by, and controls preference for, high-fat diet
    Article Snippet: .. The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs) as described in manufacturer’s instructions. .. Methylated and unmethylated plasmids were transfected into SHSY-5Y cells vector using Jetprime transfection reagent (Polyplus-transfection) and incubated for 24 h. Cells were lysed using passive lysis buffer and the activity of the enhancer assessed using a Lucia QUANTI-Luc Gold Assay #3 luciferase kit (InvivoGen).

    Article Title: The anxiety and ethanol intake controlling GAL5.1 enhancer is epigenetically modulated by, and controls preference for, high fat diet
    Article Snippet: .. The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs) as described in manufacturer’s instructions. .. Methylated and unmethylated plasmids were transfected into SHSY-5Y cells vector using Jetprime transfection reagent (Polyplus-transfection) and incubated for 24 hours.

    Polymerase Chain Reaction:

    Article Title: Sulforaphane epigenetically demethylates the CpG sites of the miR-9-3 promoter and reactivates miR-9-3 expression in human lung cancer A549 cells
    Article Snippet: .. The PCR products were digested with Nsi I and Spe I and then inserted into the pCpGfree-promoter-Lucia vector (InvivoGen, San Diego, CA, USA). .. After sequencing confirmation, the recombinant plasmid was treated with methyltransferase M.Sss I at 37°C for 2 h (New England Biolabs, Beverly, MA, USA).

    Article Title: EGR1 recruits TET1 to shape the brain methylome during development and upon neuronal activity
    Article Snippet: .. After enzymatic digestion for at least 4 h, PCR-amplified products were cloned into pCpGfree-promoter-Lucia Vector (Invivogen). ..

    Purification:

    Article Title: Epigenetic modifications but not genetic polymorphisms regulate KEAP1 expression in colorectal cancer.
    Article Snippet: People's Republic of China Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, People’s Republic of China Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, New Jersey Department of Laboratory Medicine, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, People’s Republic of China

    Luciferase:

    Article Title: YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesis.
    Article Snippet: 1Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA.. 2Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.. ✉e-mail: duojia.pan@utsouthwestern.edu The preexisting epigenetic landscape represents a barrier to oncogene-induced cellular transformation and malignancy.

    Article Title: KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2α.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Amplification:

    Article Title: YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesis.
    Article Snippet: 1Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA.. 2Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.. ✉e-mail: duojia.pan@utsouthwestern.edu The preexisting epigenetic landscape represents a barrier to oncogene-induced cellular transformation and malignancy.

    Construct:

    Article Title: KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2α.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Cloning:

    Article Title: KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2α.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Sequencing:

    Article Title: KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2α.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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